لرستان، رقم جدید دو منظوره (روغنی و کنسروی) زیتون با مقاومت مطلوب در برابر پژمردگی ورتیسیلیومی (Verticillium dahliae)

نوع مقاله : آزاد سازی رقم

نویسندگان

1 دانشیار، پژوهشکده میوه ‏های معتدله، موسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران.

2 استاد، موسسه تحقیقات گیاه پزشکی، سازمان تحقیقات آموزش و ترویج کشاورزی، تهران، ایران.

3 مربی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان لرستان، سازمان تحقیقات، آموزش و ترویج کشاورزی، لرستان، ایران بروجرد، ایران.

4 استادیار، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان قزوین، سازمان تحقیقات، آموزش و ترویج کشاورزی، قزوین، ایران.

5 استادیار، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان آذربایجان غربی، سازمان تحقیقات، آموزش و ترویج کشاورزی، ارومیه، ایران.

6 دانشیار، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان زنجان، سازمان تحقیقات، آموزش و ترویج کشاورزی، زنجان، ایران.

7 استادیار، موسسه تحقیقات اصلاح و تهیه نهال و بذر، سازمان تحقیقات آموزش و ترویج کشاورزی، کرج، ایران.

8 کارشناس، مرکز تحقیقات آموزش کشاورزی و منابع طبیعی خراسان رضوی، سازمان تحقیقات، آموزش و ترویج کشاورزی، مشهد، ایران.

9 محقق، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان زنجان، سازمان تحقیقات، آموزش و ترویج کشاورزی، زنجان، ایران.

10 کارشناس، پژوهشکده میوه ‏های معتدله، موسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران.

10.22092/rafhc.2025.365746.1341

چکیده

پژمردگی ورتیسیلیومی ناشی از قارچ Verticillium dahliae یکی از مهم‌ترین بیماری‌های زیتون در مناطق کشت این محصول در دنیا می‌باشد. مهم‌ترین و اقتصادی‌ترین روش کنترل این بیماری، استفاده از ارقام مقاوم است. منشا رقم لرستان منطقه سپیددشت استان لرستان می‌باشد. این رقم پس از ازدیاد، به همراه 109 ژنوتیپ بومی جمع‌‌آوری شده و چهار رقم تجاری به عنوان شاهد، در ایستگاه تحقیقات زیتون طارم کشت و مورد ارزیابی قرار گرفت. نتایج پنج پروژه تحقیقاتی در مدت 13 سال در خصوص ویژگی‌‌های رویشی، زایشی و مقاومت به بیماری ورتیسیلیوم نشان داد که رقم لرستان رقمی میان‌گل، دارای19/6 گل در گل‌آذین بوده و از نظر گرده‌افشانی خودسازگار است. میوه‌‌ها به فرم بیضی نامتقارن و میانگین وزن میوه 5/75 گرم است. درصد روغن در ماده خشک فرابر در پایان شهریور ماه 54/2 درصد و در آبان ماه 65 درصد اندازه‌گیری شد. قابلیت برداشت دو منظوره (کنسروی و روغنی) از ویژگی‌های مهم این رقم می‌باشد. بررسی غلظت DNA قارچ ورتیسیلیوم در کل DNA ریشه، نشان داد که رقم لرستان با دارا بودن کمترین غلظت DNA قارچ، از مقاومت مطلوبی در برابر بیمارگر برخوردار است.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

"Lorestan" a new dual purpose (oil and table) olive cultivar with good resistant to verticillium wilt (Verticillium dahliae)

نویسندگان [English]

  • A. A. Zeinanloo 1
  • H. Jafari 2
  • M. H. Koshki 3
  • M. Golmohamadi 4
  • M. Azimi 5
  • M. Taheri 6
  • F. shariati 7
  • S. H. Nazer Kakhaki 8
  • A. Abdolahi 9
  • K. Mostafavi 9
  • S. M. Tawosi 10
  • M. Dodangeh Balakhani 10
1 Temperate Fruit Research Center, Horticultural Science Research Institute, Agricultural Research Education and Extension Organization (AREEO) Karaj, Iran.
2 Professor, Iranian Research Institute of Plant Protection, (AREEO) Tehran, Iran.
3 Researcher, Lorestan Agricultural and Natural Resources Research and education Center, AREEO, Brojerd, Iran.
4 Assistant Professor, Qazvin Agricultural and Natural Resources Research and education Center, AREEO, Qazvin, Iran.
5 Assistant Professor, West Azerbaijan Agricultural and Natural Resources Research and education Center, AREEO, Urmia, Iran.
6 Associated Professor, Zanjan Agricultural and Natural Resources Research and education Center, AREEO, Zanjan, Iran.
7 Assistant professor, Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
8 Researcher, Khorasan Razavi Agricultural and Natural Resources Research and education Center, AREEO, Mashhad, Iran.
9 Researcher, Zanjan Agricultural and Natural Resources Research and education Center, AREEO, Zanjan, Iran.
10 Researcher, Temperate Fruit Research Center, Horticultural Science Research Institute, Agricultural Research Education and Extension Organization (AREEO) Karaj, Iran
چکیده [English]

Verticillium wilt caused by Verticillium dahliae is one of the most important olive diseases in the world. The most important and economical method of controlling this disease is the use of resistant cultivars. The origin of Lorestan cultivar is Sepiddasht region in Lorestan province. After the propagation of this cultivar, along with 109 native genotypes collected from 11 provinces and four varieties as controls, it was cultivated and evaluated at the Tarom Olive Research Station. The results of five research projects in a period of 13 years regarding vegetative, reproductive and resistance to verticillium disease showed that Lorestan is a mid-flowering genotype with 19.6 flowers in the inflorescence and is self-compatible in terms of pollination. It has fruits with medium-size (5.75 gr), oval-shaped and asymmetric. The percentage of oil in the dry matter of pricarp is 54.2% at the end of September and 65% at the end of November. The ability to harvest for dual purposes (table and oil) is one of the important features of this cultivar. The evaluation of verticillium fungus DNA concentration in the total root DNA showed that the Lorestan cultivar has the lowest concentration of the fungus DNA and has good resistance against the pathogen.

کلیدواژه‌ها [English]

  • Fatty acids
  • Olive germplasm
  • Oil percent
  • Disease resistant
Arias-Calderón, R., Rodriguez-Jurado, D., León, L., Bejarano-Alcazar, J., De la Rosa, R., and Belaj, A. 2015. Pre-breeding for resistance to verticillium wilt in olive: fishing in the wild relative gene pool. J. Crop Prot. 75: 25–33. doi: 10.1016/j.cropro.2015.05.006.
 
 
Arsel, H., and Cirik, N. 1994. General overview of olive breeding in Turkey. Olivae 52. 27.
 
 
Belaj, A., Muñoz-Diez, C., Baldoni, L., Satovic, Z., and Barranco, D. 2010. Genetic diversity and relationships of wild and cultivated olives at regional level in Spain. Sci. Hort. 124: 323–330. doi: 10.1016/j.scienta.2010. 01.010.
 
 
Bellini, E.1992. Behavior of some genetic characters in olive seedlings obtained by cross-breeding. Acta Hortic. 317:197–208. doi.org/10.17660/ActaHortic.1992.317.24.
 
 
Bellini, E., Giordani, E., and Parlati, M. V. 2004. The new “Arno", "Tevere" and "Basento" crossbred cultivars. Olivae 102: 42-46.
 
 
Boucheffa, S., Miazzi, M. M., Fanelli, V., and Tamendjari, A. 2016. The coexistence of oleaster and traditional varieties affects genetic diversity and population structure in Algerian olive (Olea europaea) germplasm. Genet. Resour. Crop Evol. 64: 379–390. doi: 10.1007/s10722-016-0365-4.
 
 
Cardoni, M., Quero, J. L., Villar, R., and Mercado-Blanco, J. 2022. Physiological and structural responses of olive leaves related to tolerance/susceptibility to Verticillium dahliae. Plants 11(17):2-20. doi.org/ 10.3390/ plants11172302.
 
 
Colella, C., Miacola, C., Amenduni, M., D’Amico, M., Bubici, G., and Cirulli, M. 2008. Sources of verticillium wilt resistance in wild olive germplasm from the Mediterranean region. Plant Pathol. J. 57: 533–539. doi: 10.1111/j.1365-3059.2007. 01785.
 
 
Community Plant Variety Office (CPVO). 2012. Protocol for Distinctness, Uniformity and Stability, Olive (Olea europaea L.), UPOV Code: OLEAA_EURCPVO-TP/099/1 Final.
 
 
Dabbou, S., Chaieb, I., and Rjiba, I. 2012. Multivariate data analysis of fatty acid content in the classification of olive oils developed through controlled crossbreeding. JAOCS. 89:667–674. doi.org/10.1007/s11746-011-1946-1.
 
 
Del Rio, C., Caballero, J. M., Garcia-Fernandez, M. D. 2005. Vigor (Banco de germoplasma de Cordoba). In: Variedades de olivo en Espaiia (Libro II: Variabilidad y selecciOn) (Rallo L. et al., eds). Junta de AndaluciaMAPA-Mundi-Prensa, Madrid, pp. 247-256. (in Spanish).
 
 
Fontanazza, G., Baladoni, L., and Corona, C. 1990. Obsservazioni preliminari sul valore agronomico di una nuova cultivar di olio " FS-17". Proceeding problimatiche qualitative del; olio d oliva. Accademia nazionale dell olivo. L'Olio d'oliva ed il suo futuro Spoleto 6-7 dicembre 1990. 
 
 
Green, P. S. 2002. A revision of Olea L. (Oleaceae). Kew. Bull. 57:91–140. doi: 10.2307/ 4110824.
 
 
Hartmann, H., Schnathorst, W. C., and Whisler, J. 1971. Oblonga, a clonal olive rootstock resistant to verticillium wilt. Calif. Agric. 25: 12-15.
 
 
Hoblyn, T., Grubb, N., Painter, A., and Wates, B. 1937. Studies in biennial bearing. J. Pomol.  Hort. Sci. 14(1): 39-76. doi.org/10.1080/03683621.1937.11513464.
 
 
 Hegazi, E. S., Hegazi, A. A., and Abdou M. A. 2012. Resistance of some olive cultivars to verticillium Wilt. J. Appl. Sci. Res. 8(5): 2758-2765.
 
 
International Union for the Protection of New Varieties of Plants (UPOV). 2011. Guidelines for the conduct of tests for distinctness, uniformity and stability. UPOV, TG/99/4. pp 36.
 
 
International Olive Oil Council (IOOC). 2011. Guide for the determination of the characteristics of oil olives. COI/OH/Doc. No1.
 
 
Jafary, H., Khanmohammadi, S., and Mehri, N. 2014. Detection of pathotypes of Verticillium dahliae, the causal agent of olive verticillium wilt in olive orchards of Tarom using Nested-PCR technique. JARPP. 2(2): 47-58.
 
 
Jafari, H., Zeinanloo, A. A., Taheri, M., Maleki, M., Mostafavi, K., Abodllahi, A., and Mosavi, S. S. 2016. Screening for resistance to Verticillium dahliae, the causal pathogen of olive verticillium wilt in Iranian collection of promising olive cultivars and local genotypes. (Final report) Plant Protection Research Institute, No.50292, 05.07.1395. (In Persian).
 
 
Jimenez-Diaz, R. M., Tjamos, E. C., and Ciuli, M. 1998. Verticillium wilts of major tree hosts: Olive. Pp.6- 13. In: Hiemstra J. A. and Harris D. C. (eds.), A compendium of verticillium wilts in tree species. Wageningen, Netherlands, CPRO-DLO, pp 80.
 
 
Jimenez-Diaz, R. M., Cirulli, M., Bubici, G., Jimenez-Gasco, M. M., Anoniou, P., and Tjamos, E. C. 2012. Verticillium wilt, a major threat to olive production: Current status and future prospects for its management. Plant Dis. 96:304–329. doi.org/10.1094/PDIS-06-11-0496.
 
 
Kim, K. Y., Xiao, C. L., and Rogers, D. J. 2005. Influence of culture media and environmental factors on mycelial growth and pycnidial production of Sphaeropsis pyriputrescens. Mycol. 97: 25-32. doi: 10.3852/mycologia.97.1.25.
 
 
Lavee, S. 1990. Aims, methods, and advances in breeding of new olive (Olea europaea L.) cultivars. Acta Hortic, 286:23–36.
 
 
Lavee, S., Avidan, B., Meni, Y., Haskal, A., and Wonder, M. 2004. Three new semi- dwarf table olive varieties. Olivae 102: 33-41.
 
 
López-Escudero, F., del Río, C., and Caballero, J. 2004. Evaluation of olive cultivars for resistance to Verticillium dahliae. Eur. J. Plant Pathol. 110: 79–85. doi.org/10.1023/B:EJPP. 0000010150.08098.2d.
 
 
Lopez-Escudero, F. J., and Mercado-Blanco, J. 2011. Verticillium wilt of olive: A case study to implement an integrated strategy to control a soil-borne pathogen. Plant Soil 344:1–50. doi.org/10.1007/s11104-010-0629-2.
 
 
Mahfoud, Y., Choumane, W., and Doai, F. 2018. Screening of some olive cultivars (Olea europaea L.) for tolerance to Spilocaea oleagina. IJAER. 4(2) :23-30. doi.org/10.53555/eijaer.v3i2.25.
 
 
Nankali, A., Zeinanloo, A. A., and Qanavati, F. 2007. National guidelines for the conduct of tests for distinctness, uniformity and stability in olives. Seed Registration and Certification Research Institute. Oleaa-eur/253/86/121.
 
 
Novaes, R. M. L., Rodrigues, J. G., and Lovato, M. B. 2009. An efficient protocol for tissue sampling and DNA isolation from the stem bark of Leguminosae trees. G.M.R.8: 86-96. doi: 10.4238/vol8-1gmr542.
 
 
O’Sullivan, G. 2003. Olive variety assessment for subtropical summer rainfall regions. IRDC publication no. 03/021, Rural Industries Research and Development Corporation, Barton, Australia.
 
 
Ozdemir, Y., Ozturk, A., and Guven, E. 2016. Fruit and oil characteristics of olive candidate cultivars from Turkey. Not. Bot. Horti. Agrobo. 44(1):147–154. doi:10.15835/nbha44110226.
 
 
Pérez, A. G., León, L., Sanz, C., and Rosa, R. 2018. Fruit phenolic profiling: A new selection criterion in olive breeding programs. Plant Sci. 9:241. doi:10.3389/fpls.2018.00241.
 
 
Porrassariano, A., Sorriano Martin, M. L., and Porras Piedra, A. 2003. Grafting olive cultivar Cornicabra on rootstocks tolerant to Verticillium dahliae reduces their susceptibility, J. Crop Prot. 22(2): 369-374. doi.org/10.1016/S0261-2194(02)00180-1.
 
 
Rallo, L. 1995. Selección y mejora genética del olivo en España. Olivae 59:46–53.
 
 
Rallo, L. 2014. Looking towards tomorrow in olive growing challenges in breeding. Acta Hortic. 1057:467–482. doi.org/10.17660.
 
 
Rallo, L., Barranco, D., Díez, C. M., and Rallo, P. 2018. Strategies for olive breeding: cultivated genetic resources and crossbreeding. pp. 535–600 In, Advances in plant breeding strategies: Fruits: volume 3; Al-Khayri, J.M., Jain, S.M., Johnson, D.V., Ed.; Springer International Publishing: Cham, Switzerland. 
 
 
Rodrıguez-Jurado, D., Blanco-Lopez, M. A., and Rapoport, H. 1993. Present status of verticillium wilt of olive in Andalucía (southern Spain). Bul. OEPP/EPPO 23:513–516.
 
 
Ruggeri, G. 1946. A new disease of olive. Italian Agricola 83:369-372. doi: 10.1111/j.1365-2338.1993.tb01362.x.
 
 
Sanei, S. J., Okhovat, S. M., Hejarouse, G. A., Sarami, H., and   Javan- Nikkhah, M. 2004. Olive verticillium wilt or dieback of olive in Iran. Commun. Agric. Appl. Boil. sci. 69(4): 432-442. PMID: 15756823.
 
 
Sedgley, M. 2004. Wild olive selection for quality oil production. RIRDC publication no. 04/101, Rural Industries Research and Development Corporation, Barton, Australia, pp.64.
 
 
Seifi, E., Guerin Kaiser, B., and Sedgley, M. 2015. Flowering and fruit set in olive: a review. Iran. J. Plant Physiol. 5 (2):1263-1272.
 
 
Serrano, A., Rodríguez-Jurado, D., López-Escudero, F. J., Román, B., Belaj, A., De la Rosa, R., Luque, F., and León, L. 2020. Verticillium wilt response of an olive core collection. The 7th International Horticulture Research Conference, July 1st to 30th, China, Online.
 
 
Trapero, C., Munoz-Dıez, C., Rallo, L., and Lopez-Escudero, F. J. 2011. Screening olive progenies for resistance to Verticillium dahliae. Acta Hortic. 924:137–140. doi: 10.17660/ ActaHortic.2011.924.16.
 
 
Tous, J., Romero, A., Plana, J., and Hermoso, J. F. 2002. Behavior of ten Mediterranean olive cultivars in the North-East of Spain. Acta Hortic. 586:113–116. doi.org/10.17660/ActaHortic. 2002.586.15.
 
 
Zeinanloo, A. A., Ebrahimzadeh, H., Khalighi, A., and Talaei, A. R. 2001. Study of pollination and compatibility for determination of best pollinizer for olive Cv. Zard. SpJ. 17 (2):161-172. (In Persian).
 
 
Zeinanloo, A., Shahsavari, A., Mohammadi, A., and Naghavi, M. R. 2009a. Variance component heritability of some fruit characters in olive (Olea europaea L.). Sci. Hort. 123 (1):68–72. doi: 10.1016/j.scienta.2009.07.024.
 
 
Zeinanloo, A. A., Gholami, R., Taslimpour, M. R., Ahmadi, J., Ramazani Malekroodi, M. R., Ajamgard, F., Golmohamadi, M., Azimi, M., Koshki, M. H., Rozban, M., and Fakhrodin, F. 2009b. Collection and evaluation of Iranian olive germplasm. (final report). Horticultural Science Research Institute. No 87-875-26.08.1387. (In Persian).
 
 
Zeinanloo, A. A. 2018. Evaluation and selection of superior olive genotypes with high oil and yield. IJHST. 19 (2) :171-184. (In Persian).  doi: 20.1001.1.16807154.1397.19.2.5.7.
 
 
Zeinanloo, A. A., Qaribi, K., Mostafavi, K., Abdollahi, A., and Mazhari, S. 2022. Study of flowering and pollination characteristics in native olive genotypes and cultivars of Iran. IJHST. 23(4):691-704. (In Persian). doi: 20.1001.1.16807154.1401.23.4.10.4.