Introducing the apple hybrid rootstock genotypes with an appropriate range of tolerance to fire blight disease

Document Type : Extension

Authors

1 Assistant professor, Temperate Fruits Research Center, Horticultural sciences Research Institute, Agricultural Research, Education and Extension, Karaj, Iran.

2 Associate Professor. Temperate Fruits Research Center, Horticultural sciences Research Institute, Agricultural Research, Education and Extension, Karaj, Iran.

3 Researcher, Temperate Fruits Research Center, Horticultural sciences Research Institute, Agricultural Research, Education and Extension, Karaj, Iran.

10.22092/rafhc.2025.366557.1346

Abstract

In this research, the levels of tolerance to fire blight as one of the rootstock selection indicators were investigated in ten promising apple rootstock genotypes obtained from the apple rootstock breeding program in Iran, with M9 and MM106 rootstocks as controls. for uniformity, rootstocks were propagated through cutting and their potted seedlings were inoculated in the spring of 2021 and 2022 using the branch inoculation method using a mixture of three strains of active bacteria as an inoculation medium under greenhouse conditions. Five weeks after injection, blight intensity was determined based on the ratio of the length of the dead tissue to the length of the inoculated branch (100×). Results showed that the MM106 and M9 were sensitive, the genotypes of M9OP387, AZ×M2785, B9op87 and AZ×M9185 are semi-sensitive and AZop285, Azop385, AZ×M9285, AZop286, AZop386 and Azop486 were diagnosed as semi-tolerant genotypes. AZop286, AZop386 and Azop486, with suitable vegetative reproduction by wood cutting (more than 70% rooting ability) and relative tolerance to fire blight disease, are recommended for cultivation in areas infected to fire blight disease.

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References

Afionian, M. R., and Rahimian, H. 1995. Azgil as the new host of fire blight in Iran. Summary of articles of the 12th Iranian herbal-medical congress. 11-16 Shahrivar, Karaj: 22
 
 
Aldwinckle, H. S., LoGiudice, N., Robinson, T. L., Holleran, H. T., Fazio, G., Johnson, W. C., and Norelli, J. L. 2004. Resistance of apple rootstocks to fire blight infection caused by internal movement of Erwinia amylovora from scions infections. Acta Hort., 663: 229-233.
 
 
Atashkar, D., Pirkhezri, M., and Taghizadeh, A. 2015. Production and primary evaluation of apple (Mallus domestica Borkh.) hybrid rootstocks. Iran Jour Hort Sciences., 47: 329-335. (In Farsi).
 
 
Barritt, B. H., Konishi, B. S., Dilley, M. A., and Pusey, L. 2004. Resistance of apple rootatocks to fire blight caused by Erwinia amylovora. Acta Hort., 658: 387-390.
 
 
Bastas KK. 2016. Top bacterium since 250 years: Erwinia amylovora (Burr.) Winslow et al.. Adv Plants Agr. Res., 4: 279-281.
 
 
Bogs, J., Bruchmuller, I., Erbarand, C., and Geider, K. 1998. Colonization of host plants by the fire blight pathogen Erwinia amylovora marked with genes for bioluminescence and fluorescence. Phytopathol., 88: 416-421.
 
 
Cummins, J. N., and Aldwinckle, H. S. 1983. Breeding apple rootstocks. Plant Breed Rev., 1: 294-394.
 
 
Donovan, A. 1991. Screening for fire blight resistance in apple (Malus pumila) using excised leaf assays from in vitro and in vivo grown material. Ann Appl Biol., 119: 59-68.
 
 
Ferree, D. C., and Carlson, R. F. 1987. Apple rootstocks. In: Rom RC, Carlson RF (Eds) Rootstocks for Fruit Crops, John Wiley & Sons, New York, USA, 107-143.
 
 
Gardner, R. G., Cummins, J. N., and Alswinckle, H. S. 1980. Fire blight resistance in the Geneva apple rootstock breeding program. Amer Soc Hort Sci., 105: 907-912.
 
 
Gorjizadeh, N., Khazuri, M., Damiyar, S., and Naimi, M. 2014. Reproducibility of root inoculation method and sensitivity to fire blight in some cultivars and genotypes of apple. Seedlings and Seeds., 31: 640-629.
 
 
Hassanzadeh, N., and Keshavarzi, M. 2007. Fire blight in Iran: past and current situation. Proc Int Conf Improv Fruit, Small Fruit, Nuts Vine Assortment under Present Management. Belarus, 132-135.
 
 
Johnson, W. C. 2000. Methods and results of screening for disease and insect apple rootstocks. The Compact Fruit Tree 33, pp.108-111.
 
 
Khanizadeh, S., Groleau, Y., Levasseur, A., Granger, R., Rousselle, G. L., and Davidson, C. 2005. Development and evaluation of St Jean-Morden apple rootstock series. Hort Science., 40:521-522.
 
 
Le Lezec, M., and Paulin, J. P. 1984. Shoot susceptibility to fire blight of some apple cultivars. Acta Hort. 151: 277-287.
 
 
Lespinasse, Y., and Paulin, J. P. 1990. Apple breeding program for fire blight resistance: straregy used and first results. Acta Hort., 273: 285-291.
 
 
Malki Balajo, A., Khazuri, M., Rezaei Danesh, Y., Damiyar, S., and Jafari, M. 2018. Investigation of the reaction of a number of germplasms of native Iranian apple collection to Erwinia amylovora. Jour of Seed and plant Improvement., 27: 25-36.
 
 
Norelli, J. L., Aldwinckle, H. S., and Beer, S. V. 1987. The effects of virulence of Erwinia amylovora on the evaluation of fire blight resistance in Malus. Phytopathol., 71: 1551-1555.
 
 
Norelli, J. L., Holleran, H. T., Johnson, W. C., Robinson, T. L., and Aldwinckle, H. S. 2003. Resistance of Geneva and other apple rootstocks to Erwinia amylovora. Plant Dis., 87: 26-32.
 
 
Peil, A., Emeriewen, O. F., and Khan, A. 2021. Status of fire blight resistance breeding in Malus. PlantPathol., 103: 3–12.
 
 
Van der Zwet, T., and Keil, H. L. 1979. Fire Blight: A Bacterial Disease of Rosaceous Plants. Agricultural Handbook 510, United States Department of Agriculture, Washington DC, USA, 650 pp.
 
 
Vanneste, J. L. 2001. Fire blight, the Disease and its Causative Agent. CAB International, Wallingford, Oxon OX 10 8DE, UK.
 
 
Zeller, W. 1983. Resistance of pome fruit varieties to fire blight in the Fed Rep of Germany. Acta Hort., 140: 35-42.
 
 
Research Achievements for Field and Horticulture Crops
Volume 14, Issue 1
December 2025
Pages 145-154

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History

  • Receive Date: 30 July 2024
  • Revise Date: 28 April 2025
  • Accept Date: 08 December 2025

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